This study investigates the development of advanced technologies for subsurface monitoring, with significant implications in geophysics, natural hazard mitigation, and environmental engineering. Specifically, the research focuses on the application of Distributed Acoustic Sensing using a 28 km long scientific electro-optical cable deployed in deep-sea conditions as a sensor for the detection of vibrations and mechanical disturbances along its path. DAS enables continuous, high-resolution monitoring of the underwater acoustic environment. However, the system’s sensitivity and accuracy are strongly influenced by the degree of mechanical coupling between the optical fiber and the surrounding medium, which in turn depends on both the structural characteristics of the cable and the nature of the seabed where it is installed. To address these challenges, a digital twin of the experimental configuration has been developed. This virtual model allows for the simulation and optimization of the cable’s mechanical behavior under various environmental conditions. Particular emphasis is placed on the analysis of the cable’s static equilibrium state, which is essential for understanding the actual transmission of mechanical stress to the optical fiber and for the calibration of numerical models against experimental data. The manuscript is organized as follows: Introduction, Cable Architecture, Experimental Setup, Methodology, Results, and Conclusions.