The introduction of Unmanned Surface Vessels (USVs) into maritime traffic opens opportunities for modernization of Maritime transport and reduction of incidents caused by human factors. At the same time, European guidelines for decarbonization, digitalization, and biodiversity preservation set new demands for efficiency and sustainability in the water transport sector. This study aims to determine the most important factors/criteria influencing the selection of ports for testing unmanned vessels and the experimental implementation of USV technology. Through the analysis of six ports, the weighting of criteria was assessed, maritime risks, obstacles, and conditions for integrating unmanned vessels into local infrastructure were identified. Data were collected by surveying experts from academia, public administration, and the maritime industry. The Analytic Hierarchy Process (AHP) methodology was applied as a form of multi-criteria analysis. The research results offer recommendations for decision-makers and serve as a foundation for the development of a pilot project for introducing USV systems in Maritime ports.