The success of applying for World Heritage in Macau’s historic district has brought new opportunities and challenges to the protection of Macau’s cultural heritage. Among them, Patio and BecoPatio and Beco, which used to represent the daily social and cultural life of residents, have once attracted people’s attention, but they are still not fully activated and utilized. Therefore, how to activate and develop Patio and Beco has become the focus of many studies. According to this research background, this paper puts forward the following research questions: firstly, from the perspective of the current situation and the overall system of the city, what are the characteristics of the space of Patio and Beco and what are the influence potentials of these characteristics on its subsequent activation and development? Secondly, how to comprehensively evaluate the activation potential of these spatial potentials in combination with previous studies on cultural valuesCultural value? This paper attempts to analyze the current characteristics of this low-level street by combining space syntaxSpace syntax and GISGeographic Information System (GIS), including the accessibility and openness characteristics of each Patio and Beco street at different scales and the harmony characteristics of the block related to human cognition. These characteristics are calculated by the integration degree, traversal degree, and comprehensibility degree in space syntaxSpace syntax. Finally, the quantitative results are combined with the cultural valueCultural value evaluation results made by predecessors, and the activation potential indicators of Patio and Beco streets in Macau Peninsula are obtained. On the one hand, the significance of the indicators provides the corresponding planning reference for the orderly activation of Patio and Beco, On the other hand, it also provides the corresponding method reference for the orderly protection of cultural heritage in Patio and Beco and even more cities. Through the above research, the findings of this paper can be summarized into two main points: Firstly, in terms of spatial potential, the accessibility and openness of Patio and Beco at different scales are lower than the average level of Macau city, which indicates that they are inaccessible and private spaces on the whole. However, compared with the urban scale, people are more likely to reach or cross these low-level streets from the perspective of pedestrian scale activities, and the accessibility status of Patio and BecoPatio and Beco pedestrian scale is significantly increased, even close to the average level of Macau streets. However, from the perspective of openness, Patio and Beco streets always remain introverted. From the distribution law, the accessibility of Patio and Beco streets has obvious external dependence, and its easy access is more because the external street connected with it is easier to reach. Although their openness is also influenced by the external streets, it is more related to their own forms. In addition, the block harmony related to human spatial cognition shows that most of the blocks in Patio and Beco are easy to understand, which is conducive to people’s activities in the block, but the harmony in Freguesia de São Lourenço (St. Lawrence Parish) is mostly low, which is not conducive to the activities of external people in this area. Secondly, from the perspective of activation potential, this paper obtains the activation potential scale of Patio and Beco from the perspective of cultural valueCultural value and spatial potential. The higher the activation potential is, the more priority the Patio and BecoPatio and Beco should be given and suitable for development as public commercial space or tourism cultural space, while the lower the activation potential is, the later it should be considered and suitable for use as local residential area.