Emotional volatility and difficulty in self-regulation are core characteristics of individuals with Autism Spectrum Disorder (ASD). Firstly, this study conducts color vision experiments on children with medium and high-functioning autism aged 5 to 12. Then, combined with the results of the previous experiments, a multi-sensory emotion regulation game interface based on human-computer interaction technology was designed. Through the colors and visual patterns of positive emotions, soothing music and human-computer interaction operations, an emotion soothing game of “plot narrative - visual image - color language - sound feedback - interaction mechanism” was constructed; Finally, experiments were conducted to verify the effectiveness of the emotional soothing game. Coclusions from preliminary experiments indicate that children prefer blue, green, pink and orange hues; they tend to favor high-lightness, high-purity colors, and complementary color combinations; and they show affection for round shapes, as well as human, animal, and plant images. Based on these results, the study integrates emotional design, human-computer interaction, and five-sense therapy to build an immersive game framework with the theme of “Natural Journey” around five dimensions. Players act as travelers exploring natural scenes and complete emotional connection tasks through interactions such as clicking/long-pressing rounded animal, plant images and healing tones (blue, green, pink, orange) form a visual decompression field; dynamic natural sound effects and an adaptive music system adjust audio rhythm according to interaction frequency. Effectiveness verification is conducted through wearable device pressure tests, user interviews, and observations. The results show that after the game, the average heart rate and stress values of the children have stabilized to a certain extent. Three follow-up tests confirm that the game scores high in innovation, effectiveness, interactivity, and usability on a five-point scale (average 4.5 points). Overall, the game provides positive emotional soothing experiences for children with autism, demonstrating value and significance for promotion.