This chapter explores the biomechanics of donkeys and mules, focusing on their locomotor patterns, anatomical adaptations, and interactions with saddlery, harness and terrain. While equine biomechanics is fairly well-studied in horses, research on donkeys and mules remains limited despite their global importance for draft and load carrying, particularly in low- and middle-income countries (LMICs). Donkeys are more efficient in their locomotion than horses, and gait characteristics can differ. Their different anatomical features and species-specific adaptations are likely affect movement and load-bearing capacity. The chapter also examines hoof conformation and the impact of saddlery and harness design, emphasizing the need for equipment that accommodates the distinct anatomy of donkeys and mules. By integrating existing equine research with emerging data on long-eared equids, this chapter advocates for targeted biomechanical studies to improve welfare, performance, and working conditions.