Per- and poly-fluoroalkyl substances (PFASs), a class of highly stable environmentally persistent organic compounds with multiple carbon-fluorine bonds exerts a series of negative consequences for the economy, industry, public health, and the ecosystem. It is expected that over 12,000 PFASs exist in the environment. These impacts are a global issue affecting various areas around the world, including developed and developing nations, emphasizing the need to manage emissions from different sectors across the globe. There are many current and upcoming regulations regarding the restriction of PFAS from various continents including the United States and European Union. Because PFASs are found to be highly resistant to chemical degradation strongly, the eliminations of PFASs in environmental matrixes pose a challenge to researchers and becomes subject to strict government regulations. This chapter includes the occurrences, toxicological, and ecological effects of PFASs in wastewater and the existing physicochemical remediation strategies of PFASs in wastewater. This chapter summarizes the recent advances in biological remediation strategies of PFASs with bacterial and fungal in the processes of transformation and degradation of PFASs in wastewater.