This chapter examines the strategic role of vision and mission in both general and healthcare organizations. Vision defines an organization’s aspirational future, while mission outlines its core purpose and values. Together, they guide decision-making, unify stakeholders, and shape long-term success. Drawing on examples from leading companies like Apple and Tesla, the chapter highlights how a clear, well-communicated vision and mission foster internal alignment and organizational coherence. The discussion then shifts to the healthcare sector, where these principles take on added ethical and operational importance. In healthcare, mission and vision are directly linked to patient well-being, equity, and quality of care. Through examples from Mayo Clinic and Kaiser Permanente, the chapter illustrates how healthcare leaders embed mission-driven values into structures, leadership, and daily operations. The alignment of all organizational members—from executives to frontline staff—is presented as essential for consistent, high-quality service delivery. Barriers to alignment, such as conflicting stakeholder priorities, are addressed, along with strategies to overcome them through culture, leadership, and communication. Ultimately, the chapter provides healthcare managers with a practical framework for integrating vision and mission into strategic and operational activities, reinforcing a shared sense of purpose across the organization.