Nutrition is a central determinant of wound healing and regenerative capacity. This chapter reviews how macroand micronutrients modulate inflammation, extracellular matrix formation, angiogenesis, and stem cell behavior across all phases of healing. It also integrates mechanistic insights on proteins, amino acids, lipids, vitamins, and bioactive compounds with practical strategies for assessing and optimizing nutritional status in diverse clinical settings. Special considerations for high‑risk populations—including patients with diabetes, renal disease, obesity, vascular disease, and advanced age—are highlighted. By framing nutrition as an active co‑therapy, the chapter emphasizes its essential role in improving wound outcomes and advancing regenerative medicine.