Adipose-derived stem cells (ASCs) represent a transformative tool in the evolving field of regenerative medicine, particularly in the context of wound healing. Their abundant availability, ease of harvest, and potent biological properties make them a compelling candidate for therapeutic applications in both acute and chronic wounds. This chapter provides a comprehensive overview of the biological characteristics of ASCs—including their origin in the stromal vascular fraction (SVF), surface phenotype, immunomodulatory secretome, and multipotent differentiation capacity. We examine the mechanisms through which ASCs modulate inflammation, stimulate angiogenesis, remodel the extracellular matrix, and prevent excessive fibrosis and scarring. Both preclinical and clinical studies are explored in detail, demonstrating that ASCs enhance tissue regeneration, accelerate wound closure, and improve the quality of healed skin. From diabetic ulcers to radiation-induced injuries, evidence supports their efficacy and safety, even in allogeneic applications. The chapter also delves into the challenges surrounding standardization, regulatory frameworks, and long-term safety, while highlighting the ongoing clinical trials that aim to translate this technology into routine medical practice. In synthesizing current scientific data and clinical insights, this chapter underscores the promise of ASCs as a next-generation cell-based therapy poised to redefine wound care across a range of indications.