Wound healing is a complex biological process involving multiple overlapping stages, such as hemostasis, inflammation, proliferation, and remodeling, each orchestrated through intricate cellular and molecular interactions. Preclinical models are indispensable for unraveling these processes and assessing potential therapies. Rodent models, due to their ease of genetic manipulation and low maintenance costs, remain the cornerstone for early-phase investigations, although their healing mechanism differs substantially from humans. In contrast, porcine models offer a closer anatomical and immunological resemblance to human skin, making them ideal for translational studies despite logistical and ethical challenges. Emerging technologies like 3D bioprinted skin, organoids, and microfluidic skin-on-a-chip platforms are revolutionizing preclinical research by providing physiologically relevant and ethically acceptable alternatives. Advanced imaging techniques such as Optical Coherence Tomography and multiphoton microscopy now allow non-invasive, real-time monitoring of healing, while artificial intelligence-driven analysis enhances the accuracy and reproducibility of data interpretation. Despite significant progress, issues related to reproducibility, standardization, and the limited ability of animal models to fully emulate human wound pathology continue to pose challenges. Integration of cutting-edge biotechnologies with traditional models and a focus on ethical research practices are critical for bridging the translational gap and accelerating the development of effective wound healing therapies. This review synthesizes the current landscape of preclinical wound healing research, highlighting traditional models, innovative technologies, assessment techniques, and the ongoing efforts to enhance the translational relevance of findings for clinical application.