The colon is a major organ of the gastrointestinal system. There is a good friendship between the colon, liver, pancreas, and lungs, which are vital metabolic organs. The colon becomes under pressure due to improper intake of diet, abnormal lifestyle, lack of drinking water, offensive night sleeping, mental stress, bacterial and viral infections which cause negative environment for the microbiomes. Further loss of microbiome may produce colorectal disorders like indigestion, flatulence, gastritis, irritable bowel syndrome, constipation-associated issues and further colon cancer due to aberrant expression of certain genes on abnormal signaling. Lactulose, vitamin A, vitamin E, vitamin C, and N-acetyl cysteine (NAC) have been reported to combat colorectal cancer. But the molecular mechanisms have yet to be explored against the overexpression of certain genes like KRAS G12C. Therefore, an attempt has been made in the present study to explore the biochemical mechanisms of colon cancer inhibitors such as lactulose, vitamin A, vitamin E, vitamin C, and N-acetyl cysteine having a strong affinity to bind the active site of KRAS G12C using docking as the molecular simulation.