As a reaction to the global climate crisis, the construction industry has become a key focus for carbon reduction. This work assessed carbon emissions associated with the materialization stage of buildings that had different assembly rates using a consumption-based quota method. Results showed that higher assembly rates significantly mitigated carbon emissions associated with the production of materials and construction stages. Material production remained the primary source of emissions. Additionally, prefabricated buildings demonstrated reduced carbon emissions related to labor and materials, reflecting a shift from traditional cast-in-place methods toward industrialized construction. However, machinery-related emissions tended to increase with prefabrication. Overall, the findings highlighted the capabilities of prefabricated buildings to minimize and manage carbon emissions, providing theoretical and practical support for promoting sustainability in the construction sector.