This chapter provides a comprehensive overview of oral biopsy techniques, emphasizing their critical role in diagnosing and managing oral lesions. It outlines the importance of biopsies in differentiating benign, premalignant, and malignant conditions, highlighting their impact on treatment planning and patient prognosis. The chapter details various biopsy methods—incisional, excisional, punch, fine-needle aspiration (FNAB), and brush biopsies—along with their specific indications, procedural steps, and clinical limitations. It underscores the significance of meticulous clinical examination, lesion selection criteria, and documentation to ensure diagnostic accuracy. Additionally, the chapter discusses indications and contraindications for biopsies, addressing patient safety considerations such as coagulopathies, infections, and comorbidities. Practical tips and clinical pearls are included to guide practitioners in selecting the most appropriate technique for optimal patient outcomes.