This study examines the impact of intermittent aeration on the effective removal of organics and nutrients from domestic wastewater from office building. Along with the increasing number of office buildings, the volume of domestic office wastewater produced also increases. This experiment use septic tank modification reactor, consists of 5 compartments, namely feeding tank, anoxic-separation tank, anaerobic tank, aerobic tank, and sedimentation tank. Microorganisms were used in this study to remove organic and nutrient content from wastewater. Microorganism calculation in this research is using MLSS calculation is from attached bacteria in the media. Seeding bacteria was carried out until the MLSS at kaldness media was 4000 mg/L and bioball reached 3000 mg/L. In full-day aeration, the highest COD removal efficiency was 88.24% and ammonia was 85.42%. In phase using intermittent aeration, the highest COD removal efficiency was 91.97% and ammonia was 70.45%. This reactor effluent complies with the COD limit that set by PERMEN LHK Number 68 of 2016, but not the ammonia quality standard. The largest COD removal occurred in the anoxic compartment, where COD removal from both variations decreased from about 300 mg/L to 125 mg/L. The highest ammonia decrease was in anoxic tank using full-day aeration where ammonia was decrease from about 40 mg/L to 15 mg/L. The higher COD/N ratio performs the better COD and ammonia removal in this experiment. This study shows that anaerobic-aerobic reactors require further development in treating domestic wastewater from office buildings, especially for ammonia removal, therefore the treated water can be reused.