Industrial operations lead to significant greenhouse gas emissions, with carbon dioxide being the major threat. The industrial carbon footprint is the measure of the amount of carbon dioxide released during each process of industrial production. The textile and apparel industry emits a very large amount of carbon through its processes, starting from fibre production to disposal. It has been a perennial problem, and measures are being taken to minimise the carbon footprint. One of the measures taken is the use of biotechnology. Biotechnology has been used at a great level in the textile and apparel industry as it unleashes a plethora of opportunities to reduce the carbon footprint. Biotechnology provides a variety of creative options for lowering industrial carbon footprints, such as carbon capture and utilisation of bioenergy and biofuels. Biotechnology also allows us to create more sustainable related production processes, which reduce the energy usage and greenhouse gas emissions. It can also be utilized to increase the efficiency of industrial processes, hence lowering the wastage and the pollution caused by these activities. This chapter discusses the various biotechnology-based strategies used to minimise the industrial carbon emissions generated by the textile and apparel industry. The application of biotechnology in the areas of fibres and fabrics production, textile finishing using enzymes, sustainable dyeing techniques, and bio-based recycling of textile wastes is discussed in this chapter. The chapter also divulges information on circular economy solutions through biotechnology usage to reduce industrial carbon footprint. This chapter also gives an overview of the current biotechnological measures taken in the textile and apparel industry for lowering the industrial carbon footprint and discusses the future possibilities of their usage.