Protein synthesis remains one of the most challenging competencies for students to master in life sciences. This issue is further compounded by the limited availability of teaching resources, particularly in educational institutions located in rural areas. To address this gap, the study developed an offline webpage interface aimed at conceptualizing protein synthesis and assessed its effectiveness in enhancing students’ understanding of the topic. The offline webpage interface was evaluated using the Department of Education (DepEd) Learning Resource Management and Development System (LRMDS) Evaluation Tool for Non-Print Materials, focusing on content quality, instructional design, technical design, and other findings. A pre-test and post-test design was employed to measure student learning. Ninety-four students participated in the study, utilizing the developed teaching material. The results indicated that the offline webpage interface significantly improved students’ knowledge of protein synthesis, as demonstrated by a marked increase in their post-test scores compared to their pre-test scores. Additionally, both students and teachers expressed positive perceptions of the offline webpage interface, highlighting its potential as an effective instructional tool in teaching-learning process.