In order to study the influence of seismic motion spectrum characteristics on the seismic response of high-pier, the ratio of peak acceleration to peak velocity (PGA/PGV) is selected as the characteristic parameter of ground motion spectrum, and 45 strong earthquake records with different spectral characteristics were selected to analyze the elastic-plastic time-history response of rigid frame Bridges. The effects of seismic spectrum characteristics on the displacement response, internal force response and the isolation effect of high damping rubber bearing are studied. The results show that PGA/PGV has a great influence on the seismic response of rigid frame Bridges. The displacement response, bending moment response and shear response of each pier section decrease with the increase of PGA/PGV value, and the greater the PGA/PGV value, the smaller the reduction of each response. Under different PGA/PGV ground motion, the variation range of isolation rate is wide. The correlation between pier bottom shear isolation rate and PGA/PGV is good.