Plant-based biostimulants represent a promising alternative for promoting plant growth and improving tolerance to both abiotic and biotic stress, particularly within sustainable agriculture frameworks. Several native species from the Mexican semidesert have been traditionally recognized for their bioactive properties, making them relevant candidates for the development of natural biostimulants. These plants, adapted to extreme environmental conditions, synthesize a wide variety of secondary metabolites that play key roles in regulating physiological and defense processes in cultivated plants. The application of these compounds can trigger the activation of physiological mechanisms, such as the synthesis of enzymatic antioxidants and non-enzymatic antioxidants (flavonoids, carotenoids, and ascorbic acid), which enhance the plant's ability to withstand adverse conditions and pathogen attacks. However, despite the growing interest in their agricultural application, a deeper understanding of their mechanisms of action is still needed, whether through the study of isolated phytochemicals or their synergistic combinations, in order to better comprehend their biological effects. Scientific exploration of these species could contribute not only to the development of safer and more sustainable agricultural inputs. Advancing in this field requires comprehensive research that integrates phytochemical analysis, functional studies, and validation under cultivation conditions, thus translating traditional knowledge into practical technological solutions. This would support the transition to more resilient and environmentally responsible agricultural systems while conserving the rich natural heritage of the region.