The development of bacterial biofilms that protect pathogens from conventional antimicrobial treatments is the main cause of antimicrobial resistance (AMR), a serious issue in contemporary healthcare. Because of their potent antibacterial qualities, green-synthesised silver nanoparticles (AgNPs) have demonstrated potential in breaking up biofilms. The manufacture of silver nanoparticles using an aqueous extract made from an equivalent amount of dried fruits and leaves of the popular Ayurvedic medicinal plant Phyllanthus emblica (Amalaki) is the main goal of this study. Numerous analytical methods, such as UV–visible spectroscopy, FT-IR spectroscopy, SEM, EDAX, XRD, and DLS, were used to analyze the produced AgNPs. AgNP formation was validated by UV–visible spectroscopy, and XRD analysis showed a face-centered cubic (FCC) structure with an average crystal size of 22 nm, determined by Scherrer's formula. FTIR spectroscopy identified functional groups in charge of the reduction of silver ions were found using FTIR spectroscopy. Nanoparticles between 40 and 90 nm in size were visible using scanning electron microscopy (SEM), with considerable agglomeration. A polydispersity index (PDI) of 0.287 and an average size distribution of 114.7 nm were found using dynamic light scattering (DLS). These AgNPs reduced the production of biofilms and shown potent antibacterial action against two important AMR-causing pathogens, Staphylococcus aureus and Escherichia coli. The results indicate that these nanoparticles have a lot of promise as multipurpose antibacterial agents.