Mycorrhizal association between a plant and a fungus is widespread in natural environment. Mycorrhizae are an important component of soil life and soil chemistry about 90% of the plant families are predominantly mycorrhizal. Mycorrhizal association improves water uptake and mineral nutrition absorption mainly phosphorous along with other micronutrients through the widespread surface area that enhances the absorption capability. It makes the plant strong, salinity tolerant and drought and disease resistant. Mycorrhizal biotechnology may also be used as bio fertilizer to increase the fertility of soil and is environmentally friendly. Instead of using chemical fertilizers which are cost effective and prolonged use of chemical fertilizers may leave bad impact on soil and environment. Sunflower i.e. Helianthus annus L. belonging to family Compositae (Asteraceae) is an edible oil crop introduced in Pakistan during early 1960s. Its oil is light in taste, appearance and has high vitamin E content with good fats. To check the effect of AM/VAM in comparison to other fertilizers, four different sets of experiments were performed on four different varieties of sunflower viz., Hysun 33, Hysun 38, Helico 250 and S 278. In the first experiment three different sets of treatment were setup. In the first AM/VAM spores were inoculated, in the second one cow dung (Manure) was amended, in third triple phosphate was amended while, in fourth no treatment was amended served as control. The maximum root length was observed in AM/VAM treated plants. In the third week shoot length increased over the control but maximum increase was observed in var1 and lowest in var4 in all the three treatments. Changes in the leaf area with different treatments as compared to control varied with the variety. The fresh weight and dry weight differ with different treatments showed different pattern.