Algae play crucial roles as oxygen producers and the foundation of aquatic food chains as their ecological significance. Algae are economically valuable, serving as sources of crude oil, food, and various pharmaceutical and industrial products. Red Algae's ecological significance is paramount, as they form an integral part of the food chain and contribute 40–60% of global oxygen production. Red algae species including Gelidium, Gracilaria, Pterocladiellia, Acanthopeltis, and Ahnfeltia, play essential roles in production of agar and other commercial products. The current study focuses to produce agar from the local red algae and for their enhancement alkali treatment, sonication and combine effect, biochemical analyses total carbohydrates, galactose, phenolic, sulphate and protein contents were assessed. UV–Visible spectrophotometry, FT-IR and XRD analysis were done. The results represent that among nine combinations Champia compressa, Gracilaria foliifera and Gelidium pusillum, (Hot water, NaOH and Sonication) the best results were obtained from Champia compressa, and Gracilaria foliifera with sonication treatment. However, the carbohydrate contents were 3.12, galactose content was 2.24, phenol content was 0.47 and protein contents were 1.99. The characteristics peaks were found in FT-IR and the raised levels in 2345.0 were observed for Gracilaria foliifera with sonication sample. This indicates that after the pre-treatment of hot water and alkali treatment the production of agar can be enhanced for commercial purposes.