Millions of tons organic wastes are produced annually but there is no proper disposal method that is practiced to minimize organic waste in an environmental friendly manner. That waste has harmful effects on human health and the environment. Organic wastes can be utilized in synthesizing industrial products like citric acid. A number of microorganisms have been used in fermentation studies of citric acid. Thus, present work was aimed to assess the potential of locally isolated A. tubingensis for the first time and A. niger for citric acid production. Segregated kitchen waste, crude molasses and sucrose were used as substrates. Additionally, effects of pH, carbon and nitrogen and methanol were also monitored. Findings indicate that the highest yield i.e. 16.14 ± 0.03 g/l was produced by A. tubingensis in fermentation media containing sucrose, 1.0% ammonium nitrate (AN) and 2.0% methanol while 15.97 ± 0.01 g/l was produced by A.niger in fermentation media containing sucrose, 1.0% ammonium nitrate (AN) and 1.0% methanol. Addition of methanol in fermentation media gave higher yields of citric acid with Aspergillustubingensis. Present findings demonstrated that A. tubingensis is a potential contender for the production of citric acid. Furthermore, kitchen waste could be a sustainable and cost effective source of substrate for citric acid production.