Soil health is vigorous for sustainable farming, and reinforces water retention, nutrient cycling, and microbial range. This study, part of the AM-Regen initiative in Rahim Yar Khan with the Implementing Partner WWF-PAKISTAN, Observes the impact of regenerative against conventional farming on soil health. The focus is on improving soil health indicators and safeguarding lasting sustainability over regenerative agricultural practices. A total of 92 farmers had an area of 1164 acres out of which 10 regenerative having 68 acres and 5 conventional having 10 acres, these farmers were engaged in the study, allowing an in-depth comparison of the two methods. Soil samples were together using the zig-zag technique. Regenerative applications include 100 kg/acre of vermicomposting, 80 kg/acre of Nutri-compost, and 200 kg/acre of farmyard manure. These approaches’ objective is to boost organic matter, lift microbial activity, and lessen synthetic chemicals reliance. Significant soil health indicators, such as organic matter, pH, EC, Phosphorus, and potassium levels, displayed that organic matter improved by 0.25% in regenerative farms, while conventional ranches reduced by 0.03%. Furthermore, regenerative applies balanced pH and reduced EC. The soil under regenerative practices displayed higher phosphorus and potassium levels compared to conventional approaches. Phosphorus fell to − 0.37 mg/kg under conventional practices, although it was enhanced to 0.29 mg/kg by regenerative applies. Potassium levels decreased to − 4.5 mg/kg conventionally but improved to 3.2 mg/kg under regenerative practices. These results highlight the paybacks of regenerative practices for soil health and highlight the necessity for research into its lasting effects and economic sustainability for wider implementation.