With the acceleration of industrialization, the problem of antimony and arsenic pollution in antimony mining areas has become increasingly prominent, posing a serious threat to the surrounding ecological environment and human health. The aim of this study was to prepare silica-calcium based biomineralized materials and evaluate their remediation effect on antimony and arsenic contaminated soil. The silica-calcium based materials were prepared by mixing fly ash with bentonite clay followed by ball milling and molding process and further loaded with sulfate reducing bacteria (SRB) to form biomineralized materials. The experimental results showed that the material was able to significantly reduce the mobility of Sb and As in the soil and transform them into more stable residue states. In the remediated soil, pentavalent antimony and arsenic were reduced to less migratory trivalent forms and mineral phases such as arsenopyrite (FeAsS) and pyroxene (Sb₂S₃) were generated. The formation of these minerals indicates that the silica-calcium-based biomineralized materials achieve stable solidification of antimony and arsenic through microbial-mediated reduction and precipitation. In addition, the remediation method has the advantages of environmental friendliness, low cost, and no secondary pollution, which provides a new technological pathway for the long-term treatment of antimony and arsenic contaminated soil.