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The ALCYONE project, funded under the EU Horizon Europe program, aims to develop a lab-on-chip (LoC) platform for biological studies in space environments. This study focuses on validating the system’s bioluminescence detection capability using genetically engineered Escherichia coli MG1655 cells. The cells, transformed with a luciferase-expressing plasmid, were irradiated with UV-C light for two minutes to induce stress responses, while unirradiated cells served as controls. Bioluminescence emission (520–530 nm) was measured using hydrogenated amorphous silicon (a-Si:H) photosensors integrated into the LoC platform. The experimental results confirm the system’s ability to differentiate between irradiated and control samples, demonstrating its potential for real-time, low-power monitoring of biological responses in space environments. Future work will integrate thermal control and fluidic automation to enhance biological experiment autonomy.

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Validation of On-Chip Bioluminescence Detection for Radiation-Stressed Genetically Engineered E. Coli

  • 会议论文

  • 发表日期：

    2025年

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  • Lorenzo Nardi,
  • Costanza Maria Martella,
  • Parsa Abbasrezae,
  • T. B. De Albuquerque,
  • Domenico Caputo,
  • Nicola Lovecchio,
  • Giulia Petrucci,
  • Francesca Costantini,
  • Giampiero de Cesare,
  • Daniela Billi,
  • Augusto Nascetti

摘要

The ALCYONE project, funded under the EU Horizon Europe program, aims to develop a lab-on-chip (LoC) platform for biological studies in space environments. This study focuses on validating the system’s bioluminescence detection capability using genetically engineered Escherichia coli MG1655 cells. The cells, transformed with a luciferase-expressing plasmid, were irradiated with UV-C light for two minutes to induce stress responses, while unirradiated cells served as controls. Bioluminescence emission (520–530 nm) was measured using hydrogenated amorphous silicon (a-Si:H) photosensors integrated into the LoC platform. The experimental results confirm the system’s ability to differentiate between irradiated and control samples, demonstrating its potential for real-time, low-power monitoring of biological responses in space environments. Future work will integrate thermal control and fluidic automation to enhance biological experiment autonomy.