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Pseudomonas aeruginosa (Pa) is a significant respiratory pathogen for patients with cystic fibrosis (CF), a leading cause of chronic lung infections, which are correlated with lung function decline. The actual detection methods, based on Pa microbiological identification, are time-consuming and are typically performed on expectorated sputum, that sometimes is challenging to obtain for CF treated and pediatric patients. Volatile organic compounds (VOCs) may be evaluated as indicators of Pa colonization. In this study, the aim was to characterize the VOC profile associated with Pa cultured in vitro, using a gas sensor array based on quartz crystal microbalance coated with porphyrinoids, and comparing it to that released by another common airway pathogen, such as Staphylococcus aureus. Sensor measurements were complemented by gas chromatography-ion mobility spectrometry evaluations. Results showed that both techniques are able to discriminate Pseudomonas aeruginosa.

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Characterization of Pseudomonas aeruginosa Volatilome Using a QMB-Based Gas Sensor Array for the Diagnosis of Lung Infections in Cystic Fibrosis Patients

  • 会议论文

  • 发表日期：

    2025年

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  • R. Capuano,
  • S. Ammendola,
  • F. Pacello,
  • A. Battistoni,
  • A. Catini,
  • M. Stefanelli,
  • R. Paolesse,
  • C. Di Natale

摘要

Pseudomonas aeruginosa (Pa) is a significant respiratory pathogen for patients with cystic fibrosis (CF), a leading cause of chronic lung infections, which are correlated with lung function decline. The actual detection methods, based on Pa microbiological identification, are time-consuming and are typically performed on expectorated sputum, that sometimes is challenging to obtain for CF treated and pediatric patients. Volatile organic compounds (VOCs) may be evaluated as indicators of Pa colonization. In this study, the aim was to characterize the VOC profile associated with Pa cultured in vitro, using a gas sensor array based on quartz crystal microbalance coated with porphyrinoids, and comparing it to that released by another common airway pathogen, such as Staphylococcus aureus. Sensor measurements were complemented by gas chromatography-ion mobility spectrometry evaluations. Results showed that both techniques are able to discriminate Pseudomonas aeruginosa.