The importance of disease-suppressive soils is particularly in a tropical agricultural region like Brazil where crop yields are high, and the prevalence of plant diseases caused by soilborne plant pathogens is also significantly high. Brazil is on track to achieve the largest grain harvests in history during the 2024–25 growing season with soybean and corn being the main contributors to this surge. Brazil ranks as the world’s largest producer of soybeans (166.3 million tons). This chapter discusses the role of disease-suppressive soils as the key strategy for the management of root diseases, with a specific focus on Brazil’s soybean production system. Several strategies are discussed for managing the soilborne pathogens through suppressive soils in Brazil and an important measure recognized is pathogen density by the implementation of no-till crop rotation systems in fields with a long history of soybean monoculture. Stimulation of native microbial communities, such as arbuscular mycorrhizal fungi or plant growth-promoting rhizobacteria (PGPR) is viewed as a promising strategy for enhancing soil suppressiveness. Monitoring soil suppressiveness to diseases is also discussed in detail.