Rapid Industrialization over the past few decades has resulted in the discharge of various hazardous toxic substances, especially heavy metals. Mercury is the most widely spread heavy metal and highly toxic substance, which can cause serious health-related issues for living beings. Microbial biotechnology is considered one of the emerging, innovative, cost-effective, sustainable, and eco-friendly technological tools to tackle the problem of mercury contamination compared to other conventional methods. By the process of Biosorption, microbes can remove metals depending on their properties and biomass. Environmental conditions like pH, temperature, etc., also play a significant role in this process. This study investigates the potential of the mercury resistant bacterial strain (IITISM24ID) for inorganic mercury removal at 27 °C, at 8.5 pH, and at different time intervals in the process of biosorption. The highest removal potential of IITISM24IDin in vitro conditions is found to be 80.95% for initial 15 ppm of Hg (II) concentrations. Hence, the application of the IITISM24ID bacterial strain can be effective for the removal of mercury from a mercury-stressed environment.