In the context of the diversity and complexity of laboratory processes, it is crucial to address the vulnerabilities associated with healthcare. Proper risk management becomes essential to ensure quality and safety in this environment. In this sense, the application of risk management tools and methodologies plays a crucial role in the identification, assessment and mitigation of potential risks present in laboratory processes performed, especially in a hospital environment. The present work addresses the theme of risk and safety management in a hospital environment, with the aim of promoting a safe environment for this community. The Healthcare Failure Mode and Effect Analysis methodology was applied to identify and mitigate the risks associated with medical equipment used in a medical genetics laboratory. The methodology included data collection, failure analysis, risk quantification, decision tree application and risk evaluation. Among the 19 failures analyzed none demonstrated a Risk Priority Number (RPN) greater than 8, suggesting that the equipment operates within acceptable risk thresholds. The results highlighted the importance of the safety of healthcare professionals and the proper functioning of equipment to ensure patient safety. The study contributed to the development of preventive and corrective actions, as well as providing future improvements and implementation of the methodology in other services of the hospital.