Efficient transport of cells and biomaterials is a challenge in 3D bioprinting and regenerative medicine. This study presents the design and fabrication of an innovative device for the transport of biological material, guaranteeing optimal conditions of cell viability during the process. The aim is to develop a functional, portable and controlled solution that allows cells to be kept in suspension at the right temperature and conditions until their use in bioprinting. The device was designed using 3D modelling and additive manufacturing with PLA as the base material. Electronic elements such as temperature and humidity sensors, thermal regulation systems and an autonomous power supply system using a rechargeable battery were incorporated. Functional tests were carried out in the laboratory to validate thermal stability and cell preservation at different time intervals. Tests showed that the device maintains the temperature in a controlled range (36–37 °C), suitable for cell preservation. In addition, cell viability tests confirmed that the internal environment of the device prevents dehydration and maintains the stability of the culture medium. The PLA-printed structure proved to be mechanically strong, ensuring safe and contamination-free transport. The developed device represents an efficient solution for the transport of cells in 3D bioprinting, ensuring the stability of biological conditions during the process. Its modular design and the integration of sensors allow for future improvements in connectivity and remote monitoring. This advance facilitates the implementation of bioprinting techniques in clinical and research environments, optimising the performance of bioinks and the success of tissue regeneration.