Commute trips are up to 70% of all human activity in the urban transportation system creating a significant burden. Despite an increase in the number of private cars, most of the commuting is still done by public transportation. Commuting is characterized by clear directions and short, intense periods when significant ridership needs to be served. To improve the planning of urban transport operations, it is necessary to estimate this type of travel, which usually consists of determining the O-D matrix of work trips. Thus, a generalized indicator of travel cost is proposed to use as an objective function that considers the consumption of passengers’ resources of various natures expressed in terms of cost. Along with the traditional trip indicators such as distance and time, the use of human physical energy and its economic assessment is also presented. The travel cost function is considered to fully encompass the passenger motion parameters in a public transport system and can be incorporated into an O-D matrix generation model.