Grasping the dynamics of blood flow is essential for exploring cardiovascular health and identifying irregularities. The research presents an innovative method for simulating blood circulation in microfluidic devices by combining sophisticated modeling techniques with an emphasis on mimicking arterial conditions. The research begins by creating a two-dimensional (2D) serpentine microfluidic model to analyze flow patterns and pressure distributions. The insights gained from the initial model inform the development of a three-dimensional (3D) microfluidic model, enhanced with slice simulations to accurately represent velocity fields and pressure gradients. A crucial element of the research involves developing a microfluidic device that mimics the physical and material properties of real arterial systems. The model features layers that resemble the characteristics of media and adventitia, essential for mimicking the mechanical traits of arterial walls. The final simulation includes a lifelike arterial wall constructed from myocardium material, enabling thorough analysis of velocity profiles and pressure distributions. The research effectively demonstrates the ability to replicate authentic arterial blood flow dynamics in microfluidic systems. This is validated by visual representations of velocity and pressure profiles, highlighting the intricate interactions between fluid dynamics and the mechanics of arterial walls. By connecting biological systems to computational modeling, the research establishes a robust foundation for exploring cardiovascular hemodynamics. The findings emphasize the potential of microfluidic systems for diagnostic and therapeutic applications, significantly contributing to the advancement of both research and treatment.