This study explores the impact of AI anxiety on career anxiety and career uncertainty, using Self-Determination Theory as its theoretical framework. As Artificial Intelligence (AI) continues to reshape industries, it presents both opportunities and challenges in career related factors. AI anxiety, driven by concerns over job security and skill obsolescence, affects individuals’ confidence in their career choices and decision-making processes. As a result, many individuals may experience uncertainty about their professional future. By analyzing data from 237 respondents across India, this study identifies AI anxiety as a significant factor influencing career anxiety and career uncertainty. The findings reveal that individuals with higher AI anxiety are more likely to experience higher anxiety and indecisiveness in their career. However, the overall life satisfaction helps in lowering career anxiety by providing individuals with a higher sense of well-being that counteracts the anxiety. In contrast, factors such as relatedness, competence, and AI resilience do not show significant influence on either career anxiety or career uncertainty. This study enhances the understanding of how AI anxiety shapes career-related concerns, offering insights into how individuals navigate career decisions in an AI-driven world.