This study presents the fabrication and characterization of collagen membranes obtained by electrospinning, aiming at their application in tissue engineering. Membranes were successfully produced using an eco-friendly solvent, yielding structures with homogeneous fiber distribution and defined morphology. Crosslinking was performed using glutaraldehyde and 1,4-butanediol diglycidyl ether, resulting in distinct morphological changes. Fiber morphology was assessed through scanning electron microscopy and analyzed using ImageJ. Glutaraldehyde crosslinked membranes exhibited noticeable fiber fusion and contraction, while 1,4-butanediol diglycidyl ether treatment led to a microporous architecture devoid of clear fibrous features. Gravimetric analysis confirmed porosity values and swelling behavior consistent with tissue engineering requirements. These findings support the potential of electrospun collagen membranes as customizable scaffolds for biomedical applications, particularly in tissue engineering and regenerative medicine.