Hair loss, particularly androgenetic alopecia (AGA), is a progressive condition influenced by genetic, hormonal, and environmental factors. Traditional medical treatments, such as finasteride and minoxidil, have primarily aimed at stabilizing the condition or slowing its progression, while surgical hair transplantation has provided a more definitive solution. However, both approaches have limitations, particularly regarding long-term follicular stability and patient satisfaction. This chapter explores the integration of Autologous Regenerative Therapy (ART) in hair restoration surgery, highlighting its role in improving follicular viability and enhancing the macroenvironment surrounding hair follicles. A key focus is placed on the use of adipose-derived stem cells (ADSCs) and the stromal vascular fraction (SVF), obtained through minimally invasive fat harvesting techniques like guided SEFFI, which enables the extraction of regenerative cellular components without the need for enzymatic digestion.