Polarization, as a new optical imaging tool, has been explored to assist in the diagnosis of pathology. Moreover, converting the polarimetric Mueller Matrix (MM) to standardized stained images becomes a promising approach to help pathologists interpret the results. However, existing methods for polarization-based virtual staining are still in the early stage, and the diffusion-based model, which has shown great potential in enhancing the fidelity of the generated images, has not been studied yet. In this paper, a Regulated Bridge Diffusion Model (RBDM) for polarization-based virtual staining is proposed. RBDM utilizes the bidirectional bridge diffusion process to learn the mapping from polarization images to other modalities such as H&E and fluorescence. And to demonstrate the effectiveness of our model, we conduct the experiment on our manually collected dataset, which consists of 18,000 paired polarization, fluorescence and H&E images, due to the unavailability of the public dataset. The experiment results show that our model greatly outperforms other benchmark methods. Our data and code are available at https://github.com/xiaoyu-z/RBDM/

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Diffusion-Based Virtual Staining from Polarimetric Mueller Matrix Imaging

  • Xiaoyu Zheng,
  • Jing Wen,
  • Jiaxin Zhuang,
  • Yao Du,
  • Jing Cong,
  • Limei Guo,
  • Lin Luo,
  • Chao He,
  • Hao Chen

摘要

Polarization, as a new optical imaging tool, has been explored to assist in the diagnosis of pathology. Moreover, converting the polarimetric Mueller Matrix (MM) to standardized stained images becomes a promising approach to help pathologists interpret the results. However, existing methods for polarization-based virtual staining are still in the early stage, and the diffusion-based model, which has shown great potential in enhancing the fidelity of the generated images, has not been studied yet. In this paper, a Regulated Bridge Diffusion Model (RBDM) for polarization-based virtual staining is proposed. RBDM utilizes the bidirectional bridge diffusion process to learn the mapping from polarization images to other modalities such as H&E and fluorescence. And to demonstrate the effectiveness of our model, we conduct the experiment on our manually collected dataset, which consists of 18,000 paired polarization, fluorescence and H&E images, due to the unavailability of the public dataset. The experiment results show that our model greatly outperforms other benchmark methods. Our data and code are available at https://github.com/xiaoyu-z/RBDM/