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We fabricated two different models of apertures to improve the point spread function in a confocal microscope. The first has a cascaded linear-conic (L/C) distribution. The second has a hexagonal annulus modulated with a central disc of a linear distribution. We computed the point spread function (PSF) using the fast Fourier transform (FFT) corresponding to the two fabricated apertures. For the first model, we compared the PSF results with the corresponding PSF for black and white (B/W) apertures. For the second model, we compared the PSF results with the PSF in the case of a transparent hexagonal shape. This arrangement of (L/C) aperture is equivalent to a triangular shape, which is useful to improve the PSF compared to the rectangular aperture, since its width is twice that of the rectangular aperture. Finally, we reconstructed the image from the input object in the confocal plane of the confocal laser scanning microscope (CLSM) provided with the above-modulated apertures.

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Design of an Improved Aperture and an Application in Microscopy

  • 章节

  • 发表日期：

    2025年

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  • Abdallah Mohamed Hamed

摘要

We fabricated two different models of apertures to improve the point spread function in a confocal microscope. The first has a cascaded linear-conic (L/C) distribution. The second has a hexagonal annulus modulated with a central disc of a linear distribution. We computed the point spread function (PSF) using the fast Fourier transform (FFT) corresponding to the two fabricated apertures. For the first model, we compared the PSF results with the corresponding PSF for black and white (B/W) apertures. For the second model, we compared the PSF results with the PSF in the case of a transparent hexagonal shape. This arrangement of (L/C) aperture is equivalent to a triangular shape, which is useful to improve the PSF compared to the rectangular aperture, since its width is twice that of the rectangular aperture. Finally, we reconstructed the image from the input object in the confocal plane of the confocal laser scanning microscope (CLSM) provided with the above-modulated apertures.