The article is devoted to the study of the influence of the field rotation frequency in the working chamber of the electromechanical transducer with a discrete secondary part on the efficiency of cavitation effect on microorganisms. It serves as a foundation for further scientific research in the application of cavitation. The study highlights the importance of understanding the dynamic processes involved in cavitation for optimizing industrial applications and improving the efficiency of sterilization processes. Methods of liquid treatment require thorough elaboration in order to improve energy efficiency and environmental safety of sterilization processes. The authors use an innovative approach consisting in the creation and control of cavitation bubbles by means of an electromechanical transducer, where the field rotation frequency plays an essential role. This system has significantly higher energy efficiency as well as processing speed. The conducted experiments allow to reveal optimal conditions for maximum impact on microorganisms, which opens prospects for application of this method in various industries, including medical disinfection and water treatment. The paper considers both theoretical and practical aspects of the influence of rotation frequency, analyzes the mechanisms of cavitation formation and its effect on cellular structures. The results obtained indicate the significant potential of this technology to achieve a high level of deactivation of microorganisms with minimal energy and resource consumption. Abstract This article is intended for professional researchers in the field of biotechnology and engineering and can serve as a basis for further scientific research in the field of cavitation application.