Integrating bio-enzymes into the detergent industry represents a transformative approach towards sustainable entrepreneurship against traditional chemical-based cleaning agents. With increasing environmental concerns and the demand for greener solutions, bio-enzymes are an effective method for minimizing the ecological impact of detergents by removing stains at lower temperatures and decreasing energy consumption. Furthermore, the development of innovative business models that utilize bio-enzyme technology and incorporate waste utilization aims to commercialize eco-friendly products in response to the increasing consumer demand for sustainable alternatives. By embracing circular economy principles, this research not only contributes to environmentally sustainable goals but also opens new avenues for economic growth within the industry, highlighting the importance of interdisciplinary approaches in fostering sustainable entrepreneurship. This paper outlines the environmental and economic benefits of bio-enzyme detergents, innovative business models, and waste utilization highlighting their potential to revolutionize the industry while promoting eco-friendly practices.