This study focuses on the detection and assessment of risks associated with manual load handling, with an emphasis on last-mile logistics services. Combining the Technical Guide for the Assessment and Prevention of Risks Related to the Manual Handling of Loads, developed by the Spanish National Institute for Safety and Health at Work (INSST), with advanced simulation tools, this research proposes an innovative approach to risk evaluation. Data collection activities, theoretical analysis using the technical guide, and digital simulation with SIEMENS Tecnomatix software were conducted to determine the tolerability of theoretical risks, followed by the discussion and validation of results through simulation. This work highlights the utility of simulation as a key tool to optimize risk assessment processes, providing detailed results that involve theoretical frameworks. The research lays the groundwork for the prevention of musculoskeletal injuries and the integration of Ergonomics 5.0 principles into workplace safety and operational efficiency.