In this chapter, we present how computational modelling can be used as an additional tool for working in tissue engineering and regenerative medicine. The ability to use computational models in combination with other technologies, such as in vitro and in vivo experiments, is very useful because it provides the opportunity to study many different conditions from different perspectives, obtaining different data that can be compared and integrated to achieve a deep understanding of the biological mechanisms involved. Moreover, computer simulations allow to reduce the number of in vivo experiments, providing us with an alternative methodology. As it will be shown here, computational predictions can assist in final design decisions in bioreactors, microfluidic systems, or organ-on-chip devices, in optimizing culture conditions, or in estimating the long-term behavior of engineered tissues in vivo. Here, several modelling strategies are shown to present its potential applicability in tissue engineering and regenerative medicine.