Introduction: COVID-19 infection is one of the most important current challenges globally. Data show that COVID-19 infections among health workers are higher than those of the general population. The aim of this study was the translation and the adaption of the questionnaire that was piloted to evaluate the level of health and safety of health professionals in Greece after the outbreak of the pandemic. Methods: A structured forward-backward translation process was performed. The authors cooperated with a strategic sample of experts. The pilot study was conducted in three public hospitals of Attica, during the period September to December 2021. The selection of the participants was based on random sampling. The research tool that was used was the questionnaire on “Health and safety of health workers in COVID-19”. Data were evaluated using Cronbach’s alpha reliability coefficient, intraclass correlation coefficient, t-test, and ANOVA test. Results: Cronbach’s α was 0.846, showing high internal consistency. The intraclass correlation coefficient was 0.969, indicating high test–retest reliability. There was a statistically significant gender difference in the scale relevant to health and safety risks and gender. Also, a statistically significant difference was found between the prevention measures and between the hospitals as well. Conclusions: Comprehension of the Greek version of the original questionnaire was achieved. It seems to be a promising tool with acceptable internal consistency. Further study on a larger sample is required to generalize the results.