This chapter gives an overview of the development and application of paper-based substrates for surface-enhanced Raman spectroscopy (SERS), a powerful analytical technique that leverages the enhancement of Raman signals to detect low-concentration analytes. The chapter begins by introducing the fundamental principles of SERS, highlighting the role of nanostructured surfaces in enhancing the Raman scattering signal. It then explores the paper substrate, with its unique combination of accessibility, cost-effectiveness, and versatility, which has emerged as a promising substrate for SERS applications. A brief review of the various design strategies and fabrication methods for paper-based SERS substrates is presented, detailing the advantages and limitations of each approach. The chapter further discusses the diverse applications of paper-based SERS substrates in fields such as bioanalysis, food safety, and environmental monitoring, emphasizing their potential for point-of-care diagnostics. Finally, a brief perspective on the future directions of paper-based SERS substrates is provided.