The aquatic environment is known to be seriously contaminated by antibiotic residues on a worldwide basis. In the world, antibiotics are widely used as a major class of pharmaceutically active chemicals (PhACs). Manures from animal husbandry, hospitals, municipal sewage, and home disposal are the main sources of these wastes. Surface and drinking waters, sewage effluents, soils, sediments, and a variety of plant species in nations including China, Japan, South Korea, Europe, the United States, Canada, and India are all often found to contain these residues. A significant amount of antibiotics are released into the environment as metabolites in urine and feces, despite their usage as medications in both people and animals. As sensitive and quantitative analytical methods have improved, antibiotics at quantities ranging from nanograms per liter (ng/L) to milligrams per liter (mg/L) have been regularly detected in environmental matrices. Because agricultural soils have such high particle adsorption capabilities, they are a major reservoir for antibiotic residues. There are major worries about the potential for human and animal intake of these medications due to the fact that plants cultivated in soils irrigated with water tainted with PhAC can absorb and deposit them in a variety of tissues, including roots, leaves, and fruits. This research concludes with an assessment and synopsis of commercially available and spin-off antibiotic detection sensors, highlighting their portability and versatility, through elucidating the state, function, and prospects of electrochemical biosensors in antibiotic detection.