Micro-autologous Fat Transplantation (MAFT), introduced in 2007, represents a transformative advancement in fat grafting for aesthetic and reconstructive surgery. Rooted in precise fat parcel size delivery principles and inspired by pioneers like Coleman and Carpaneda, the MAFT technique emphasizes micro-droplet injections of 1/60–1/240 mL to minimize central necrosis and enhance graft survival. Central to this innovation is the MAFT-GUN®, a patented instrument that ensures uniform, controlled delivery of adipose tissue. This chapter highlights the MAFT technique’s unique strengths: its ability to optimize fat viability through strategic parcel size control, ensuring enhanced vascularization, minimal trauma, and consistent long-term results. The technique has demonstrated exceptional efficacy in facial contouring, breast augmentation, and regenerative applications, enabling precise restoration of volume loss and tissue defects. The MAFT-GUN®, available in manual and automated versions, supports surgeons by increasing precision, reducing procedural variability, and improving patient outcomes. By combining clinical evidence with technological ingenuity, MAFT has addressed challenges such as graft unpredictability, central necrosis, and integration issues, solidifying its role in modern plastic and reconstructive surgery. This chapter provides an evidence-based, detailed exploration of MAFT, offering insights into its mechanisms, applications, and implications for the future of regenerative medicine.