This chapter explores the principles, methodologies, and advancements in the cryopreservation of adipose tissue. We will discuss the biological characteristics that influence cryopreservation, techniques to optimize protocols, and the translational hurdles that must be overcome for broader clinical adoption. Additionally, emerging technologies, such as mechanical pre-processing, will be highlighted as innovative approaches to enhance the cryopreservation process. Together, these insights aim to provide a comprehensive understanding of this critical aspect of regenerative medicine.