Biodiesel is increasingly recognized as a promising alternative to reduce dependence on non-renewable fossil fuels, offering an environmentally friendly solution for partial or total replacement of oil and gas in combustion engines and energy generation. One of its significant advantages is that it does not require new investments in infrastructure or engine retrofitting. However, using edible oils for biodiesel production can affect food safety and increase production costs. To address this issue, waste oils have emerged as a viable, cost-effective non-food alternative for biodiesel production. This study aims to map the current research on biodiesel production from waste oil via the enzymatic route using bibliometric techniques. By analyzing trends, leading journals, and key research areas, with a focus on Distillers Corn Oil (DCO) a coproduct from the corn-ethanol industry, the study provides insights into this evolving field. The methodology involved searching for relevant articles in the Web of Science and Scopus databases, following established inclusion and qualification criteria. A complementary search was also conducted to expand the sample. The bibliometric analysis revealed a consistent increase in publications over the years, with 2021 being particularly notable. The study identified the most influential journals, frequently used keywords, and the network of interactions between authors, shedding light on current trends and research interests and hubs in biodiesel production. Key challenges, including the reliance on edible oils and environmental impacts, were discussed, enhancing the advantages of the enzymatic route over traditional chemical processes. The enzymatic route is highlighted for its higher efficiency and lower environmental impact, offering benefits such as high purity biodiesel, milder reaction conditions, lower energy requirements, reduced capital costs, and less wastewater production. Despite these advantages, the enzymatic process faces challenges, notably the high cost of enzymes and the need for longer reaction times to achieve high conversion rates. Ongoing research is focused on developing solutions to reduce enzyme costs and improve the efficiency of the process. The study underscores the importance of DCO as a valuable non-food feedstock for biodiesel and its potential synergies with the corn-ethanol industry. The findings offer a strong foundation for future research and development in biodiesel production. Future work should focus on developing more efficient and economical technologies, especially for biodiesel production from corn oil-based feedstocks, and assessing the environmental and socio-economic impacts of this production. This study also contributes to a comprehensive understanding of the current state of research in enzymatic biodiesel production from waste oil and identifies opportunities for future advancements.