Bacteriocins are antimicrobial peptides ribosomally synthetized by bacteria, effective against bacteria, fungi and viruses. They can be classified into two classes based on the presence or absence of post-translational modifications, or into four classes based on size, heat stability, and structural complexity. These peptides eliminate bacteria via formation of membrane pores, membrane depolarization, disturbing glucose uptake, interfering enzymatic reactions in cell wall synthesis or affecting DNA, RNA and protein synthesis. Efficiency of bacteriocin delivery to the site of action and achievement of therapeutic effect are largely influenced by its solubility, permeability and stability profiles. Over the past years, innovative (nano)technological platforms for bacteriocin delivery have been increasingly investigated to overcome the limitations associated with the use of free bacteriocins. The most frequently used (model) bacteriocin in this type of studies is nisin. This chapter brings overview of state of the art of bacteriocin delivery systems aimed for different routes of administration or evaluated only in terms of antimicrobial activity in vitro. Future perspectives of bacteriocin delivery and therapeutic effect are highlighted to boost the further investigations in the field.