Natural entities possess special features that are difficult to recreate with synthetic materials. Various bioinspired and biomimetic delivery systems such as cells, pathogens, and top-down systems, including exosomes and natural cell-membrane derived nanosystems, have been deeply investigated for drug delivery applications in cancer, neurological disorders, and infections. The effective strategy of biomimicry enhances nanotherapeutics by preserving and expressing structural and functional self-recognition markers, allowing for more effective drug delivery systems that maximize therapeutic potential at the target site while reducing adverse effects. Cell-derived nanocarriers replicate cellular structures, optimizing interactions with specific cells or tissues with remarkable accuracy and mitigating systemic side effects, thereby overcoming the limitations of traditional synthetic nanomaterials. They protect drugs from degradation, enhance stability and bioavailability, ensure prolonged circulation, and enable controlled release, maintaining therapeutic concentrations over time. Fabricating and characterizing biomimetic cell-based nanocarriers involves several intricate challenges, including design principles, fabrication, purification, and characterization. Robust protocols are required to preserve the intricate organization of membrane proteins and lipids from the cell source while maintaining their functional integrity. Also, it is fundamental to properly characterize their properties including their ability to accurately mimic native cellular structures and retain their bio-functions and targeting capabilities.