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This study aimed to investigate the potential of biohydrogen production from palm oil mill effluent (POME) through anaerobic fermentation in a batch reactor, under pH 5, with POME pretreated by heat at 80 ℃ for 50 min. The bacteria Paraclostridium bifermentans (P. bifermentans), Clostridium butylicum (C. butylicum) and Bacillus Amyloliquefaciens (B. Amyloliquefaciens) were employed to compare the efficiency of biohydrogen production. The experiment comprised four conditions: 1) POME + Inoculum (control), 2) POME + Inoculum + P. bifermentans, 3) POME + Inoculum + C. butylicum and 4) POME + Inoculum + B. Amyloliquefaciens. The experimental results indicated that the condition with C. butylicum supplementation achieved the highest hydrogen production, reaching 53.38%, compared to the control group and the conditions supplemented with P. bifermentans and B. Amyloliquefaciens, which produced 36.28%, 40.22% and 15.65%, respectively. According to COD, TS, TSS, and TVS results, it showed that all the experiments could reduce the organic contaminants from POME effluent and converted to biohydrogen energy. Although the results indicated a trend of C. butylicum enhancing biohydrogen production under optimal conditions, this study suggests microbial related to biohydrogen production could be explored to further improve hydrogen production efficiency in future research.

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Potential of Biohydrogen Production from Palm Oil Mill Effluent

  • 会议论文

  • 发表日期：

    2025年

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  • Sirinthra Yurapak,
  • Suthida Sawatphakdi,
  • Siriya Duangsupa,
  • Kamonrat Kanhom,
  • Yanisa Laoong-u-thai

摘要

This study aimed to investigate the potential of biohydrogen production from palm oil mill effluent (POME) through anaerobic fermentation in a batch reactor, under pH 5, with POME pretreated by heat at 80 ℃ for 50 min. The bacteria Paraclostridium bifermentans (P. bifermentans), Clostridium butylicum (C. butylicum) and Bacillus Amyloliquefaciens (B. Amyloliquefaciens) were employed to compare the efficiency of biohydrogen production. The experiment comprised four conditions: 1) POME + Inoculum (control), 2) POME + Inoculum + P. bifermentans, 3) POME + Inoculum + C. butylicum and 4) POME + Inoculum + B. Amyloliquefaciens. The experimental results indicated that the condition with C. butylicum supplementation achieved the highest hydrogen production, reaching 53.38%, compared to the control group and the conditions supplemented with P. bifermentans and B. Amyloliquefaciens, which produced 36.28%, 40.22% and 15.65%, respectively. According to COD, TS, TSS, and TVS results, it showed that all the experiments could reduce the organic contaminants from POME effluent and converted to biohydrogen energy. Although the results indicated a trend of C. butylicum enhancing biohydrogen production under optimal conditions, this study suggests microbial related to biohydrogen production could be explored to further improve hydrogen production efficiency in future research.